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The multi-cultural site Łosino 15 (AZP 10-29/39), Kobylnica commune, pomorskie voivodeship, revealed the acquired material of the Pomeranian culture and the Wielbark culture. The majority of the discovered features are to be connected with the first settlement horizon. The features of the Pomeranian culture in the number of 135 were dominated by pits and hearths. The collection of ceramics included...
The purpose of this study was to assess whether Taxol-induced changes in microtubular dynamics are accompanied by apoptosis. CHO AA8 cells were treated with different Taxol concentrations (0.25μM, 0.5μM, 1μM) for 24h. The effects of Taxol exposure were analyzed using fluorescence microscopy and flow cytometry (TUNEL and annexin V-FITC/propidium iodide assays). 0.25μM Taxol caused the appearance of...
Directed motility of the cell is strictly dependent on regulation of the assembly/disassembly of actin filaments. New formed „daughter” filaments press against the membrane, thus generating a protrusive force, which is necessary for the initial stage of the movement. Precisely ordered geometry of „dendritic actin brush” results from the action of the Arp2/3 complex i. e. actin polymerization. The...
Many cellular events are directly related to the cytoskeleton’s dynamics, which remain under control of the protein regulatory system. Proteins of the ADF/cofilin family have been shown to be active in depolymerizing F-actin, as well as severing actin filaments and creating free barbed ends. Their various functions are regulated, among others, by phosphorylation, pH, the state of the nucleotide bound...
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