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AimsThe aim of this study was to develop and evaluate cross‐priming amplification (CPA) for the detection of avian reovirus (ARV).
Methods and ResultsFive specific primers were designed, on the basis of the σNS sequence of the S1133 ARV strain. Incubation temperature and primer concentrations were determined. The optimal incubation conditions in a water bath were 61·3°C for 45 min. No reverse transcription...
The aim of the study was to determine the effect of field strain, serotype 7 (FAdV-7 JN-5/10), of fowl adenovirus infection on the replication of Rispens/CVI988 strain of Marek's disease virus. Ninety one-day-old SPF chickens were divided into six groups. The chickens from group I were vaccinated against Marek's disease (MD) and 24 h later infected with the adenovirus; chickens from group II were...
The study describes successful isolation of 96 fowl adenovirus (FAdV) strains from 789 chickens from 95 flocks. PCR specific for hexon gene encoding L1 loop was conducted. Amplicons were subjected to sequence analysis. The sequences were analysed by the software: BLAST, Geneious 6.0, and MEGA 5, then aligned with different adenovirus strain reference sequences accessible in GenBank database. The examined...
The aim of the study was to determine the influence of simultaneous infection of chicken embryo fibroblasts (CEF) with different doses of adenovirus field strain serotype 7 (FAdV-7 JN-5/10j) and turkey herpesvirus strain FC 126 (FC 126 HVT) on replication of the herpesvirus in in vitro cultures. Three experiments were performed: simultaneous infection of CEF with adenovirus and HVT; inoculation of...
The strains of adenoviruses were isolated from 356 birds with clinical form of Marek's disease and coinfection with adenoviruses. A hexon gene fragment coding loop LI of adenovirus strains was sequenced and obtained data were analysed with BLAST, Geneious 5.3, and MEGA5 software by comparison with nucleotide sequences of reference strains of fowl adenoviruses (FAdV-1 - FadV-12), two turkey adenoviruses,...
Two thousand one hundred and forty birds belonging to 39 different species from different locations in Poland were examined. The study has taken place from the early spring till late autumn 2007-2010 when the activity of the mosquitoes was the highest. The brain samples were taken from the birds and whole cellular RNA was isolated, then the RT-PCR and NRT-PCR were performed to detect the presence...
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