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Surgically obtained early sheep embryos collected at morula, blastocyst, expanded blastocyst or hatching/ hatched blastocyst developmental stages were subjected to vitrification. The experiment was carried out by the traditional vitrification method, employing 0.25 ml insemination straws as carriers of embryos. The vitrification medium (VM) based on a modified phosphate-buffered solution (PB1) containing...
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