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The presented studies were concentrated on construction of the recombinant mammalian expression vector - pSecTag2B, carrying canine parvovirus VP2 gene and its N-terminal fragment. Evaluation of safety of experimental vaccines was done on laboratory animals and immunogenic properties were tested on dogs. CPV specific antibodies were not detected by HI test in serum samples collected from dogs vaccinated...
Neorickettsia helminthoeca is the etiological agent of „salmon poisoning disease”. The fluke - Nanophyetus salmincola - is a vector for neorickettsia which requires three hosts for the completion of its life cycle. The first intermediate host is a stream snail - Oxytrema silicula. Fish are the second host in which cercariae encyst. The definitive hosts are fish-eating mammals such as dog, raccoon,...
The aim of these studies was to attempt to infect dogs by their inoculation with cultured Borrelia burgdorferi isolated from ticks in Poland and to use PCR and N-PCR to evaluate the spread of infection. Polish strain 236/2 of B. burgdorferi isolated from Ixodes ricinus and cultured in BSK-H medium (passage 4 and 5) was used for dog inoculation. Throughout the experimental trial all dogs, including...
In our studies two primer pairs within the VP2 protein gene of canine parvovirus were chosen on the base of the sequence of Polish isolates. The sensitivity of detection of canine parvovirus in stool samples by Nested PCR was increased 60% in comparison with the standard PCR method and it was 30% higher than the virus isolation in tissue culture. A simple procedure of sample preparation with the use...
The studies on the influence of bacteria and parvovirus infection on reproductive failure in silver and blue foxes were carried out on 21 Polish farms. Parvoviral antibodies were detected in 35 out of 229 sera tested. Parvovirus infection could be the main cause of small litter size in only 6 farms An abundant growth of E. coli, Staphylococcus aureus, Streptococcus pyogenes, Proteus vulgaris and Clostridium...
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