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Rab7 is a small GTPase that plays a crucial role in the regulation of transport from early to late endosomes and lysosomes, phagosome maturation and in lysosomal biogenesis in mammalian cells. It contains conserved and unique sequence elements that mediate its function. Two Rab7 genes, Rab7a (703 bp) and Rab7b (707 bp) were identified in the unicellular eukaryote Paramecium by PCR amplification. They...
RT-PCR and Northern blot analysis were performed in order to search for a putative beta-adrenergic receptor (β-AR) in Paramecium using several β2-adrenergic-specific molecular probes.Under strictly defined RT-PCR conditions DNA species of expected molecular size about 360 bp were generated with the primers corresponding to the universal mammalian β2-AR sequence tagged sites (located within the 4th...
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