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A series of cytotoxic neutral dicarboxylatoplatinum(II) complexes containing D(+), L(-) or DL-malate dianion and ethylenediamine or 1-ethylimidazole as ligands were examined using ATPase activity assays and the proton extrusion test. ATPase activity assays in vitro on plasma membrane H+- ATPase and on mitochondrial ATPase were carried out. The concentrations of compounds inhibiting enzyme activity...
A series of 30 Saccharomyces cerevisiae aci+ mutants (characterized as acidifying Ogur's glucose medium containing bromocresol purple) were isolated after EMS mutagenesis. All the mutants excreted acid metabolites to the medium after 24 or 48 hours of incubation. The character of the aci+ mutations was defined using classical genetic techniques. Three of the aci+ mutants were studied by molecular...
Quaternary ammonium salts inhibited the growth of yeast especially at pH higher (pH 8) than optimal. It was postulated that compounds integrate with the cell membrane and interfere with its functions. The yeast cell ultrastructure investigated under an electron microscope confirms this hypothesis. A relatively high percentage of cells treated at pH 6 with the quaternary ammonium salt of alanine derivative...
A quaternary ammonium salt, dodecyloxycarboxymethyl N, N, N - trimethyl ammonium chloride (IM), inhibits glucose-stimulated proton extrusion in yeast cells. The IM-resistant mutant (IMR) and pmal mutant are less sensitive to this inhibition. The inhibition of amino acids uptake by IM depends on repressed or derepressed conditions. IM treatment of yeast cells leads to a change of internal pH in a quinacrine...
Population dynamics in batch and continuous culture of Shigella flexneri 3b lysogenic for plaque-less phage X and sensitive to this phag lb serovars have been determined. The abrupt change in the fraction of lysogens resulting from the death, especially of sensitive cells, was observed. The death should result of spontaneous liberation of phage X as concluded from the growth dynamics of the sensitive...
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