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To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies, but a system in which SE or ORG can be induced alternatively in the same...
In Arabidopsis thaliana, in vitro culture of immature zygotic embryos on medium supplemented with 2,4-D results in formation of somatic embryos via direct embrogenesis (DSE). The analysis of the nature of signals/stimuli involved in determination of embryogenic response in cultured explants can reveal genetic and physiological mechanisms involved in plant embryogenesis. The key factors for DSE induction...
In Arabidopsis biotechnology plants are regenerated in vitro via shoot organogenesis induced in callus derived from different somatic tissues. An alternative way of in vitro plant regeneration via somatic embryogenesis has not been applied in Arabidopsis so far. Recently, it was found that development of Arabidopsis somatic embryos can be induced in the culture of immature zygotic embryos and that...
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