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The mannitol transporter EII mtl from Escherichia coli is responsible for the uptake of mannitol over the inner membrane and its concomitant phosphorylation. EII mtl is functional as a dimer and its membrane-embedded C domain, IIC mtl , harbors one high affinity mannitol binding site. To characterize this domain in more detail the microenvironments of thirteen residue positions...
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