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We have utilized tau‐assembled and tau‐stabilized microtubules (MTs), in the absence of taxol, to investigate the effects of tau isoforms with three and four MT binding repeats upon kinesin‐driven MT gliding. MTs were assembled in the presence of either 3‐repeat tau (3R tau) or 4‐repeat tau (4R tau) at tau:tubulin dimer molar ratios that approximate those found in neurons. MTs assembled with 3R tau...
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