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Control of pH in aminoethylation reactions is critical for maintaining high selectivity towards cysteine modification. Measurement of aminoethylation rate constants by liquid chromatography mass spectrometry demonstrates reaction selectivity of cysteine≫amino-terminus≫histidine. Lysine and methionine were not reactive at the conditions used. For thiol modification, the acid/base property of the γ-thialysine...
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used to sequence modified oligonucelotides (MONs). Under delayed pulsed ion extraction conditions, sequence ions of MONs resulting from fragmentation within the ion source can be observed. In this work, several common types of antisense MONs with sizes up to 25-mer were studied including an oligodeoxynucleotide...
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