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Monoclonal antibodies (mAbs) are a class of proteins belonging to the immunoglobulin family that function by stimulating the body's immune system to identify and destroy compromised cells, malignant proteins, or pathogens, such as bacteria or viruses. A basic mAb production and purification process is presented in this chapter. The template process can be broken down into five areas: upstream; clarification;...
To perform their various functions, protein surfaces often have to interact with each other in a specific way. Usually, only parts of a protein are accessible and can act as binding sites. Because proteins consist of polypeptide chains that fold into complex three‐dimensional shapes, binding sites can be divided into two different types: linear sites that follow the primary amino acid sequence and...
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