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Membrane tubular structures are important communication pathways between cells and cellular compartments. Studying these structures in their native environment is challenging, due to the complexity of membranes and varying chemical conditions within and outside of the cells. This work demonstrates that a calcium ion gradient, applied to a synthetic lipid nanotube, triggers lipid flow directed toward...
In article number 1703541, Tatsiana Lobovkina and co‐workers demonstrate that the application of a calcium ion gradient to the surface of a synthetic lipid nanotube induces movement of lipids in the direction of increasing calcium ion concentration, resulting in the formation of a membrane‐filled prolate lipid aggregate. This “bulge” can be translated without direct physical contact, by moving a calcium...
Here, we report on a novel protocol for determining the viability of individual cells in an adherent cell culture, without adversely affecting the remaining cells in the sample. This is facilitated using a freestanding microfluidic perfusion device, the Multifunctional Pipette (MFP), which generates a virtual flow cell around selected single cells. We investigated the utility on four different cell...
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