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Two anchoring filament (AF)-associated proteins, uncein and laminin-5, have been described. Of these, uncein is the only one which by antibody studies appears to be abnormally expressed in all forms of JEB. Uncein is composed of 3 subunits (165, 130, & 100 kD), and binds exclusively to AFs on the epidermal side of salt split (SS) skin; of the two uncein subunits examined by 2-D gel electrophoresis,...
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