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To explore oxidative damage induced by PBDE 209 and its mechanism we carried out this research. Mice were given intragastrically with PBDE 209 via a metal gastric-tube at different levels (0, 50, 100, 200 mg/(kg·d)) for seven days and the malondialdehyde (MDA) content, superoxide dismutase (SOD) activities in liver and liver index were mensurated. Results showed that along with elevation of PBDE 209...
In order to study the effect of butyl benzyl phthalatee (BBP) on hepatic cells of rat, hepatic cells of rat were exposed to different dose (5 μmol / L, 20 μmol / L, 80 μmol / L) of BBP in vitro for 1 hour. Thiobarbituric acid (TBA) method , KCl-SDS precipitation method and Dinitrophenylhydrazone (DNPH) colorimetric method were used to detect malondialdehyde (MDA) content, DPC coefficient changes and...
To investigate the oxidative damage induced by di-(2-ethylhexyl) phthalate (DEHP) and analyze the possible function of such effects. This study took Esisenia foelide as experimental material to detect the levels of DNA-protein crosslinks (DPC), the contents of malondialdehyde (MDA), and the activity of surperoxide dismutase (SOD). The Esisenia foelide were exposed to DEHP for 2 hours in vivo with...
To study the effect of Butylbenzyl phthalate inducing DNA damages in Rat lung cells. KCl-SDS assay and comet assay were used to reveal the amount of DNA-protein crosslinks and effect of DNA damages induced by different doses of Butylbenzyl phathalate in vitro. The results showed that Butylbenzyl phathalate could cause DNA breakage at low level (10 μmol/L, P<;0.01), while at higher level (40 μmol/L,...
Owing to its good flame-retardant effect, polybrominated diphenyl ethers (PBDEs) were widely added to electronic, electrical appliances, as well as textiles and chemical products. Decabromodiphenyl Ether (BDE-209) is one class of PBDEs which was most demanded in international market. To illustrate the possible function of BDE-209 on rat liver, hepatic cells were expose to BDE-209 with different concentrations...
In this study, the gene encoding purine nucleoside phosphorylase (PNPase) from Bacillus subtilis W168 was identified, cloned and expressed in Bacillus subtilis AG208. The gene encodes a polypeptide of 233 amino acids with a calculated molecular weight of 25,018 Da. The enzyme activity of the recombinant protein (AGPNP) was analyzed by temperature and pH perturbation difference spectra. Results showed...
To illustrate the possible function of genotoxicity of BBP on DNA, this study took mice livers as experimental materials to detect the levels of DNA-protein crosslinks (DPC), the activity of surperoxide dismutase (SOD), and the contents of malondialdehyde (MDA) with intraperitioneal injection of different doses of BBP for two consecutive 14 days. DPC levels in hepatic cells showed a dose-dependent...
How does inhaled formaldehyde transfer the genotoxicity from exposure organ to site distant organs via blood? This is the key question to understand leukemia induced by formaldehyde. To answer this question, we used new born calf serum (NBS) as "blood" and Hela cells as materials to investigate its effects on cellular thiol concentration and DNA-protein crosslink (DPC) induced by formaldehyde...
The objective of this study is to explore the relation between oxidative stress and DNA-protein crosslink formation caused by formaldehyde (FA) inhalation in mice liver. 15 Kun Ming mice were divided into 3 groups: control group, 3.0 mg/m3 gaseous FA inhaled group, 3.0 mg/m3 gaseous FA and lipoic acid (LA) treated group. The content of reduced glutathione (GSH) and the content of DPC were measured...
The purpose of the present study was to examine levels of mRNA for DNA methyltransferases (DNMTS) and methyl- CpG-binding proteins 2 (MeCP2 ) in the lung of mouse after middle-level gaseous formaldehyde exposure. Male mice were exposed to clean air or 3.0 mg/m3 formaldehyde for consecutive 72 h and the lungs were dissected for RNA extraction. The mRNA levels of dnmtl, tin in t3a and mecp2 gene were...
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