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We constructed a new cell line which stably expressed Ca v 3.1 and Kir2.1 subunits in HEK293 cells (HEK293/Ca v 3.1/Kir2.1) in order to investigate the unknown cellular signaling pathways of T-type voltage-dependent calcium channels. The new cell line has a stable resting membrane potential and can activate T-type Ca 2+ channels by KCl-mediated depolarization. We showed that...
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