We constructed a new cell line which stably expressed Ca v 3.1 and Kir2.1 subunits in HEK293 cells (HEK293/Ca v 3.1/Kir2.1) in order to investigate the unknown cellular signaling pathways of T-type voltage-dependent calcium channels. The new cell line has a stable resting membrane potential and can activate T-type Ca 2+ channels by KCl-mediated depolarization. We showed that Ca v 3.1 activation resulted in the level of p21 ras -GTP in the cells being rapidly decreased during the first 2 min, and then recovering between 2 min and 15 min. The kinetics of ERK activation following Ca v 3.1 stimulation was also investigated. ERK activation was decreased from 2 min to 5 min after KCl stimulation, which means that Ca v 3.1 activation reduced ERK activity in the very early stages of activation. In addition, similar results for Ca v 3.1 activation were also shown in the case of Sos1, Grb2, and Shc, which means that Ca v 3.1 activation triggers p21 ras and that this signal is transferred to ERK by Sos1, Grb2, and Shc.