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The current study aims for a simple, rapid, and smartphone‐based enhanced chemiluminescence (eCL) technique for sensitively detecting glucose in human urine. Herein, 1,3‐propanedithiol cross‐linked gold nanoparticles (GNPs‐PDT) via a simple reduction method is synthesized. Further, it is characterized by UV–vis spectroscopy and transmission electron microscopy. The synthesized GNPs‐PDT is found to...