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Maintenance of the rod-like structure of potato spindle tuber viroid (PSTVd), which contains over 20 loops and bulges between double-stranded helices, is important for viroid biology. To study tolerance to modifications of the stem-loop structures and PSTVd capacity for mutation repair, we have created 6 mutants carrying 3–4 nucleotides deletions or insertions at three unique restriction sites, EagI,...
Transgenic plants offer a low‐cost approach for the production of pharmaceutically important and commercially valuable recombinant proteins. Our studies were focused on the plant‐based production of human interleukin 2 (hIL‐2) and its fusion with proteinase inhibitors, either SPI2 from Galleria mellonella or CMTI from Cucurbita maxima. Finally, five plant expression cassettes were obtained. Three...
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