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Establishment and characterization of two cobia, Rachycentron canadum, cell lines derived from cobia brain (CB) and cobia fin (CF) are described. Caudal fin and brain from juvenile cobia were dissociated for 30 and 10 min, respectively, in phosphate‐buffered saline containing 0.25% trypsin at 25 °C. The optimal culture condition for both dissociated cells (primary cell culture) was at 28 °C in Leibovitz‐15...
Magnetic Resonance Spectroscopy (MRS) is a non-invasive technique in which biochemical information can be obtained in vivo. LCModel and QUEST are two quantization tools for magnetic resonance spectroscopy. In this paper, a comparison between LCModel and QUEST is presented. After a description of the methods, we compared their performance on quantification of dominant metabolites in the brains of a...
The purpose of this study was to explore the time course of cerebral lactate concentration in ischemic rat brain after sacrifice, in which area the morphology signal was still normal in the early phase. Ischemic rat model was used in this study. Eighty rats were fasted overnight before sacrifice and divided into 16 groups randomly. Lactate concentration was measured by using LD kit. Results showed...
The purpose of this study was to investigate whether the Central Nervous System (CNS) analgesics affect the metabolites of swine brain by nasal spraying. Single voxel proton magnetic resonance spectroscopy (1H-MRS) was performed on 9 swine. The data were post-processed using LCModel software. Before nasal spraying butorphanol tartrate, the mean Glutamate compounds (Glx) concentration was 9.276+0.542...
We propose a computational framework for learning predictive image features as "biomarkers" for Alzheimer's disease discrimination using high-resolution magnetic resonance (MR) brain images. We focus on the exploration of a very large (>500 million) feature space derived extensively from the deformation and tensor fields. In such a huge space, our computational tool supports an automatic...
To investigate the accuracy for quantification of brain total creatine (Cr) concentration using in vivo long echo time (TE) PRESS sequence with an external standard and LCModel. Ten swine and an external standard containing a detectable compounds of known concentration were studied by using 1.5 T GE Signa scanner and the standard head coil; the single-voxel proton magnetic resonance spectroscopy (...
To investigate the accuracy for quantification of brain total creatine (Cr) concentration using in vivo long echo time (TE) PRESS sequence with an external standard and LCModel. Ten swine and an external standard containing a detectable compounds of known concentration were studied by using 1.5 T GE Signa scanner and the standard head coil; the single-voxel proton magnetic resonance spectroscopy (...
The control of neuron position and orienting axon outgrowth is a significant focus in the development of cell biosensors and the study of neural regeneration. During the development, neurons communicate with each other by growing out axons and dendrites to form network. For the central nerve system: brain and spinal cord, which are very limited in their ability to repair. A better resolution for the...
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