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The present study intended to develop receptor‐mediated luciferase reporter gene assays to evaluate and compare the estrogen receptor (ER), androgen receptor (AR) and thyroid hormone receptor (TR) activities of target chemicals. Di‐2‐ethylhexyl‐phthalate (DEHP), chlorpyrifos (CPF), 2,4‐dichlorophenoxyacetic acid (2,4‐D) and bisphenol A (BPA) are some of the most common contaminants in drinking water...