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Aims: To characterize the function of both metK1‐sp (sp1190) and metK2‐sp (sp1566) in vitro and in vivo, and to study the regulation of doxorubicin production by overexpressing the metK.
Methods and Results: We cloned two orfs into pET32a(+) respectively, and the formation of S‐Adenosyl‐l‐methionine was clearly observed in the in vitro enzyme assays as functional MetKs. Reverse transcriptase polymerase...