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Human β‐defensin 3 (hBD‐3) activates antigen‐presenting cells through Toll‐like receptors (TLRs) 1/2. Several TLR1/2 agonists have been identified but little is known about how they might differentially affect cellular activation. We compared the effects of hBD‐3 with those of another TLR1/2 agonist, Pam3CSK4, in human monocytes. Monocytes incubated with hBD‐3 or Pam3CSK4 produced interleukin‐6 (IL‐6),...