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The biosynthetic pathway for the branched‐chain amino acids is present in plants, fungi and bacteria, but not in animals, making it an attractive target for herbicidal and antimicrobial drug discovery. Ketol‐acid reductoisomerase (KARI; EC 1.1.1.86) is the second enzyme in this pathway, converting in a Mg2+‐ and NADPH‐dependent reaction either 2‐acetolactate or 2‐aceto‐2‐hydroxybutyrate to their...
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