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Thymidine kinase 1 (TK1) is an important cancer biomarker whose serum levels are elevated in early cancer development. We developed a microchip electrophoresis immunoaffinity assay to measure recombinant purified TK1 (pTK1) using an antibody (Ab) that binds to human TK1. We fabricated PMMA microfluidic devices to test the feasibility of detecting Ab–pTK1 immune complexes as a step toward TK1 analysis...