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An amperometric aptasensor has been developed by immobilization of DNA aptamer with Neutral Red (NR) on polycarboxylated thiacalix[4]arene onto the electropolymerized NR layer at a glassy carbon electrode. The NR reduction current recorded after 10 min incubation decayed with increased thrombin concentration due to limitation of the electron exchange in the surface layer. The aptasensor makes it possible...