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The recombinant single-chain trispecific antibody against three toxins including Okadaic acid (OA), saxitoxin (STX) and tetrodotoxin (TTX) was developed. Three single-chain variable fragment (ScFv) genes were linked via linkers (Gly4-Ser)3 to construct fusion ScFv gene OST-ScFv through splicing by overlapping extension. Fusion gene OST-ScFv was 2 256 bp, encoding 752 amino acids, and was inserted...
The high occurrence rate of the cardiovascular diseases and the development of proteomics bring the cardio-muscle proteomics. All have already opened a related research one after another at home and abroad currently, but almost of these focuses on the cardiovascular disease, few on sports science. According to the importance of heat in sports, applying cardio-muscle proteomics on sports science is...
This paper presents unprecedented accomplishments toward unidirectional guidance of cellular migration via durotaxis-based microtopography. In contrast to previous efforts, micropost arrays of varying anisotropy (muPVAs) optimize unidirectional control of cell migration through dual axis durotaxis cues which restrict movement in the lateral direction in addition to promoting migration in the direction...
It is a difficult problem of studying the protein folding process in the protein research field. In this paper, a new simulation algorithm-snake simulation algorithm is put forward to solve the problem. In order to prove the key function in snake simulation algorithm is correct and feasible, theory of molecule oscillation and molecule kinetics are used. In addition, bring forward a model that describes...
It is a difficult problem of studying the protein folding process in the protein research field. In this paper, a new simulation algorithm-snake simulation algorithm is put forward to solve the problem. In order to prove the key function in snake simulation algorithm is correct and feasible, theory of molecule oscillation and molecule kinetics are used. In addition, bring forward a model that describes...
Fibronectin is patterned on silicone rubber surfaces in arrays of 2 mum diameter dots using contact removal method. Cells are cultured on the patterned surface and their migration and the structure of actin cytoskeleton were studied.
A major goal of tissue engineering is to generate living cellular constructs with 3-D, tissue-like organization of cells and matrices. The generation of mechanical forces by the cellular cytoskeleton plays a critical role in the organization of matrix and of cellular colonies. The anchorage of the cytoskeleton to a substrate is essential for cellular tractional processes. We show that placing cells...
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