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We report the synthesis, spectroscopic characterization, molecular docking and biological evaluation of nine pyrazino‐imidazolinone derivatives. These derivatives were evaluated for their anticancer activity against three cancer cell lines: 518A2 melanoma, HCT‐116, and HCT‐116 p53 knockout mutant colon carcinoma. The MTT assay was employed to assess their effectiveness. Among the nine compounds tested,...