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A simple and sensitive method using in vivo microdialysis coupled with UPLC–MS/MS was established to evaluate the pharmacokinetics of Shuang Hu tincture (SHZTN). Xevo TQ‐S was used to analyze the active ingredients of mesaconitine, hypaconitine, 4‐hydroxycinnamic acid, ferulic acid and N‐(2, 3‐dimethyl phenyl)‐2‐ aminobenzoic acid of SHZTN. Samples were prepared using a methanol precipitation method...