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Dictyostelium discoideum phenylalanine hydroxylase (DicPAH; residues 1–415) was expressed in Escherichia coli and purified for structural analysis. Apo DicPAH and DicPAH complexed with dihydrobiopterin (BH2) and FeIII were crystallized using 0.06 M PIPES pH 7.0, 26%(w/v) PEG 2000 by the hanging‐drop vapour‐diffusion method. Crystals of apo DicPAH and the DicPAH–BH2–FeIII complex diffracted to 2.6...