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In article number 1908299, Marcy Zenobi‐Wong and co‐workers introduce a novel two‐photon patterning strategy based on enzymatic couplings, nonfouling hydrophilic photocages, and improved protein functionalization reagents. Using time‐ and spatially controlled laser exposure, precise anchoring of growth factors within a hydrogel can guide the axons along biochemical paths. Illustration: Axon guided...
Three‐dimensional (3D) control over the placement of bioactive cues is fundamental to understand cell guidance and develop engineered tissues. Two‐photon patterning (2PP) provides such placement at micro‐ to millimeter scale, but nonspecific interactions between proteins and functionalized extracellular matrices (ECMs) restrict its use. Here, a 2PP system based on nonfouling hydrophilic photocages...
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