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Plant breeders constantly need to adapt their research to the ever-changing market needs and agricultural practices. To achieve these goals, they need to competently combine different genetically-governed characters in a genotype, this is a complex, time-consuming and labour intensive task. In modern plant breeding, molecular markers are of increasing importance, and it is today undeniable that their...
The breeding companies and laboratories involved in this article cover a wide range of crops grown in the temperate climate zone: small grain cereals, oilseed crops, forage crops, turf, vegetables and potato. Speed and efficiency are becoming increasingly important in variety breeding and doubled haploids (DH) and genetic markers are important biotechnological tools to accelerate materials to market...
A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was...
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