Search results for: Olivia McAuliffe

Items from 1 to 20 out of 21 results

article

The use of bacteriophages to control and detect pathogens in the dairy industry

Lisa O'Sullivan, Declan Bolton, Olivia McAuliffe, Aidan Coffey

International Journal of Dairy Technology > 73 > 1 > 1 - 11

Dairy products are susceptible to bacterial contamination during both primary production and the processing stages. Pasteurisation and high hydrostatic pressure are used to eliminate bacteria from milk; however, some heat‐tolerant pathogens and bacterial spores may survive these processes. Bacteriophages (phages) are viruses that infect and lyse specific bacteria. These or their encoded proteins may...

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Comparative genomics of Cp8viruses with special reference to Campylobacter phage vB_CjeM_los1, isolated from a slaughterhouse in Ireland

Lisa O’Sullivan, Alan Lucid, Horst Neve, Charles M. A. P. Franz, more

Archives of Virology > 2018 > 163 > 8 > 2139-2154

Campylobacter phage vB_CjeM_Los1 was recently isolated from a slaughterhouse in the Republic of Ireland using the host Campylobacter jejuni subsp. jejuni PT14, and full-genome sequencing and annotation were performed. The genome was found to be 134,073 bp in length and to contain 169 predicted open reading frames. Transmission electron microscopy images of vB_CjeM_Los1 revealed that it belongs to...

article

Comparative genomic and metabolic analysis of three Lactobacillus paracasei cheese isolates reveals considerable genomic differences in strains from the same niche

Ewelina Stefanovic, Olivia McAuliffe

BMC Genomics > 2018 > 19 > 1 > 1-13

Background Strains of Lactobacillus paracasei are present in many diverse environments, including dairy and plant materials and the intestinal tracts of humans and animals. Their adaptation to various niches is correlated to intra-species diversity at the genomic and metabolic level. In this study, we compared the genome sequences of three L. paracasei strains isolated from mature Cheddar cheeses,...

article

Whole genome sequence analysis; an improved technology that identifies underlying genotypic differences between closely related Listeria monocytogenes strains

Edward M. Fox, Aidan Casey, Kieran Jordan, Aidan Coffey, more

Innovative Food Science & Emerging Technologies > 2017 > 44 > C > 89-96

As the new technology of whole genome sequencing (WGS) has been shown to have greater discriminatory power in differentiating strains than the much-used pulsed-field gel electrophoresis (PFGE), there is currently a transition from using PFGE to WGS for disease outbreak investigation. Therefore, there is a need for comparison of bacterial isolates using both PFGE and WGS. In this study, two pairs of...

article

Strains of the Lactobacillus casei group show diverse abilities for the production of flavor compounds in 2 model systems

Ewelina Stefanovic, Anne Thierry, Marie-Bernadette Maillard, Andrea Bertuzzi, more

Journal of Dairy Science > 2017 > 100 > 9 > 6918-6929

Cheese flavor development is directly connected to the metabolic activity of microorganisms used during its manufacture, and the selection of metabolically diverse strains represents a potential tool for the production of cheese with novel and distinct flavor characteristics. Strains of Lactobacillus have been proven to promote the development of important cheese flavor compounds. As cheese production...

article

Advances in the genomics and metabolomics of dairy lactobacilli: A review

Ewelina Stefanovic, Gerald Fitzgerald, Olivia McAuliffe

Food Microbiology > 2017 > 61 > C > 33-49

The Lactobacillus genus represents the largest and most diverse genera of all the lactic acid bacteria (LAB), encompassing species with applications in industrial, biotechnological and medical fields. The increasing number of available Lactobacillus genome sequences has allowed understanding of genetic and metabolic potential of this LAB group. Pangenome and core genome studies are available for numerous...

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Characterisation of the antibacterial properties of a bacterial derived peptidoglycan hydrolase (LysCs4), active against C. sakazakii and other Gram-negative food-related pathogens

Lorraine Endersen, Aidan Coffey, R. Paul Ross, Olivia McAuliffe, more

International Journal of Food Microbiology > 2015 > 215 > Complete > 79-85

Illness caused by the consumption of contaminated food products continues to represent one of the main challenges facing food manufacturers worldwide. Even with current intervention technologies and increased hygiene measures, foodborne illness remains a significant threat to public health. This coupled with the increasing emergence of multidrug resistant pathogens has increased the need for the development...

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Assessment of wild non-dairy lactococcal strains for flavour diversification in a mini-Gouda type cheese model

Daniel Cavanagh, Kieran N. Kilcawley, Maurice G. O'Sullivan, Gerald F. Fitzgerald, more

Food Research International > 2014 > 62 > Complete > 432-440

Wild lactococci possess enhanced metabolic capabilities in comparison to industrial dairy strains, including increased amino acid-converting enzyme activities. A bank of Lactococcus lactis strains isolated from different non-dairy environments exhibited wider carbohydrate fermentation profiles in comparison to dairy lactococcal strains. In addition, these non-dairy lactococci had the ability to ferment...

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Characterization of plant-derived lactococci on the basis of their volatile compounds profile when grown in milk

Debebe Alemayehu, John A. Hannon, Olivia McAuliffe, R. Paul Ross

International Journal of Food Microbiology > 2014 > 172 > Complete > 57-61

A total of twelve strains of lactococci were isolated from grass and vegetables (baby corn and fresh green peas). Ten of the isolates were classified as Lactococcus lactis subsp. lactis and two as Lactococcus lactis subsp. cremoris based on 16S rDNA sequencing. Most of the plant-derived strains were capable of metabolising a wide range of carbohydrates in that they fermented D-mannitol, amygdalin,...

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Isolation and characterisation of six novel mycobacteriophages and investigation of their antimicrobial potential in milk

Lorraine Endersen, Aidan Coffey, Horst Neve, Olivia McAuliffe, more

International Dairy Journal > 2013 > 28 > 1 > 8-14

Using Mycobacterium smegmatis as a rapidly growing host, six mycobacteriophages were isolated from a variety of environmental sources and characterised by restriction endonuclease digestion. All six phages had long, non-contractile tails and isometric heads characteristic of Siphoviridae and were relatively heat-stable up to 72 °C and generally retained infectivity at pH4–pH10 for 60 min. Each individual...

article

Genome sequence of the phage clP1, which infects the beer spoilage bacterium Pediococcus damnosus

David Kelly, Orla O'Sullivan, Susan Mills, Olivia McAuliffe, more

Gene > 2012 > 504 > 1 > 53-63

Pediococcus damnosus (P. damnosus) bacteriophage (phage) clP1 is a novel virulent phage isolated from a municipal sewage sample collected in Southern Ireland. This phage infects the beer spoilage strain P. damnosus P82 which was isolated from German breweries. Sequencing of the phage has revealed a linear double stranded DNA genome of 38,013 base pairs (bp) with an overall GC content of 47.6%. Fifty...

article

A study of the prevalence of methicillin-resistant Staphylococcus aureus in pigs and in personnel involved in the pig industry in Ireland

Marianne Horgan, Yvonne Abbott, Peadar G. Lawlor, Angela Rossney, more

The Veterinary Journal > 2011 > 190 > 2 > 255-259

To evaluate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the pig population in Ireland, nasal swabbing was employed in three abattoirs to screen 440 pigs from 41 geographically distributed farms. One hundred individuals involved in the pig industry were also nasally screened. No MRSA isolates were recovered from the pigs and only two of the humans tested were identified...

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Assessment of Escherichia coli O157:H7-specific bacteriophages e11/2 and e4/1c in model broth and hide environments

Brid Coffey, Lucia Rivas, Geraldine Duffy, Aidan Coffey, more

International Journal of Food Microbiology > 2011 > 147 > 3 > 188-194

The efficacy of bacteriophages e11/2 and e4/1c as potential biocontrol agents for Escherichia coli O157:H7 in food applications was assessed under conditions relevant to the food chain environment. The stability of each phage was determined following exposure to varying environmental conditions (pH, temperature, water activity, and sodium chloride) and the ability of each phage to infect and reduce...

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Genome analysis of the Clostridium difficile phage ΦCD6356, a temperate phage of the Siphoviridae family

Marianne Horgan, Orla O'Sullivan, Aidan Coffey, Gerald F. Fitzgerald, more

Gene > 2010 > 462 > 1-2 > 34-43

The temperate phages ΦCD6356 and ΦCD6365 were isolated and characterised following mitomycin C induction of 43 Clostridium difficile strains. Both phages belong to the Siphoviridae family and have genome sizes of 37,664bp for ΦCD6356 based on sequence data and approximately 50kb for ΦCD6365 based on restriction analysis. Protein analysis revealed similar protein profiles and indicated posttranslational...

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Cloning and expression of a mureinolytic enzyme from the mycobacteriophage TM4

Marine Henry, Máire Begley, Horst Neve, Fiona Maher, more

FEMS Microbiology Letters > 311 > 2 > 126 - 132

In this study, we describe the characterization, cloning, expression and purification of the lysin A gene of the mycobacteriophage TM4. The gene TM4_gp29 (gp29) is a 1644‐bp gene that codes for a 58.6‐kDa protein and contains peptidoglycan recognition protein, Zn‐binding and amidase catalytic domains. The gene was cloned into Escherichia coli using the ‘His‐Tag’ pQE60 vector. After affinity chromatography‐mediated...

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In silico analysis of Ardmore, a novel mycobacteriophage isolated from soil

Marine Henry, Orla O'Sullivan, Roy D. Sleator, Aidan Coffey, more

Gene > 2010 > 453 > 1-2 > 9-23

Ardmore is a novel mycobacteriophage isolated from a soil sample collected in County Waterford, Ireland. The genome of this phage has been fully sequenced and is composed of 52,141 bp of linear double stranded DNA with a GC content of 61.49%. 88 ORFs were identified of which 34 were assigned predicted functions based on their homology to previously characterised proteins, their location in the genome,...

article

Genome of a virulent bacteriophage Lb338-1 that lyses the probiotic Lactobacillus paracasei cheese strain

Debebe Alemayehu, R. Paul Ross, Orla O'Sullivan, Aidan Coffey, more

Gene > 2009 > 448 > 1 > 29-39

There is a lack of fundamental knowledge about the influence of bacteriophage on probiotic bacteria and other commensals in the gut. Here, we present the isolation and morphological and genetic characterization of a virulent narrow-host-range bacteriophage, φLb338-1. This phage was isolated from fresh sewage and was shown to infect the probiotic cheese strain Lactobacillus paracasei NFBC 338. Electron...

article

The presence of pMRC01 promotes greater cell permeability and autolysis in lactococcal starter cultures

Vincenzo Fallico, Olivia McAuliffe, Gerald F. Fitzgerald, Colin Hill, more

International Journal of Food Microbiology > 2009 > 133 > 3 > 217-224

Conjugative transfer of plasmid-associated properties is routinely used to generate food-grade derivatives of lactococcal starter strains with improved technological traits. However, the introduction of one or more plasmids in a single strain is likely to impose a burden on regular cell metabolism and may affect the growth characteristics of the transconjugant culture. The aim of this study was to...

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Genome analysis of the obligately lytic bacteriophage 4268 of Lactococcus lactis provides insight into its adaptable nature

Maeve Trotter, Olivia McAuliffe, Michael Callanan, Rob Edwards, more

Gene > 2006 > 366 > 1 > 189-199

Analysis of the complete nucleotide sequence of the lactococcal phage 4268, which is lytic for the cheese starter Lactococcus lactis DPC4268, is presented. Phage 4268 has a linear genome of 36,596 bp, which is modularly organised and encompasses 49 open reading frames. Putative functions were assigned to approximately 45% of the predicted products of these open reading frames based on sequence similarity...

article

Lantibiotics: structure, biosynthesis and mode of action

Olivia McAuliffe, R.Paul Ross, Colin Hill

FEMS Microbiology Reviews > 2001 > 25 > 3 > 285-308

The lantibiotics are a group of ribosomally synthesised, post-translationally modified peptides containing unusual amino acids, such as dehydrated and lanthionine residues. This group of bacteriocins has attracted much attention in recent years due to the success of the well characterised lantibiotic, nisin, as a food preservative. Numerous other lantibiotics have since been identified and can be...

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Search results for: Olivia McAuliffe

The use of bacteriophages to control and detect pathogens in the dairy industry

Comparative genomics of Cp8viruses with special reference to Campylobacter phage vB_CjeM_los1, isolated from a slaughterhouse in Ireland

Comparative genomic and metabolic analysis of three Lactobacillus paracasei cheese isolates reveals considerable genomic differences in strains from the same niche

Whole genome sequence analysis; an improved technology that identifies underlying genotypic differences between closely related Listeria monocytogenes strains

Strains of the Lactobacillus casei group show diverse abilities for the production of flavor compounds in 2 model systems

Advances in the genomics and metabolomics of dairy lactobacilli: A review

Characterisation of the antibacterial properties of a bacterial derived peptidoglycan hydrolase (LysCs4), active against C. sakazakii and other Gram-negative food-related pathogens

Assessment of wild non-dairy lactococcal strains for flavour diversification in a mini-Gouda type cheese model

Characterization of plant-derived lactococci on the basis of their volatile compounds profile when grown in milk

Isolation and characterisation of six novel mycobacteriophages and investigation of their antimicrobial potential in milk

Genome sequence of the phage clP1, which infects the beer spoilage bacterium Pediococcus damnosus

A study of the prevalence of methicillin-resistant Staphylococcus aureus in pigs and in personnel involved in the pig industry in Ireland

Assessment of Escherichia coli O157:H7-specific bacteriophages e11/2 and e4/1c in model broth and hide environments

Genome analysis of the Clostridium difficile phage ΦCD6356, a temperate phage of the Siphoviridae family

Cloning and expression of a mureinolytic enzyme from the mycobacteriophage TM4

In silico analysis of Ardmore, a novel mycobacteriophage isolated from soil

Genome of a virulent bacteriophage Lb338-1 that lyses the probiotic Lactobacillus paracasei cheese strain

The presence of pMRC01 promotes greater cell permeability and autolysis in lactococcal starter cultures

Genome analysis of the obligately lytic bacteriophage 4268 of Lactococcus lactis provides insight into its adaptable nature

Lantibiotics: structure, biosynthesis and mode of action

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