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Vitamins are organic foods that are indispensable for the growth and health of the body. In case of health problems, especially lack of vitamins, people are more trusting doctor and nutritionist to find out whether the treatment is still low-level disorder or chronic. With the use of technology applications such as expert systems, the needs of society in diagnosing early vitamin deficiency can be...
Single nucleotide polymorphisms (SNPs) are the most common genetic variations that can be genotyped effectively by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Although PCR-RFLP has become the popular technique, search for available restriction enzymes and design of feasible primers for SNP genotyping is still a challenging task. An available restriction enzyme at...
Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) is an important technique in basic research of complex genetic diseases associated with single nucleotide polymorphisms (SNPs). Performing PCR-RFLP for SNP genotyping requires feasible primer pairs subject to numerous constraints and, in turn, requires a restriction enzyme to discriminate the target SNP. However, designing...
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is useful in small-scale basic research studies of complex genetic diseases that are associated with single nucleotide polymorphism (SNP). Designing a feasible primer pair is an important work before performing PCR-RFLP for SNP genotyping. However, in many cases, restriction enzymes to discriminate the target SNP resulting...
The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique is often used in laboratories and many basic research studies of complex genetic diseases associated with single nucleotide polymorphisms (SNP). When performing PCR-RFLP for SNP genotyping, feasible primer pairs are subject to numerous constraints and require a restriction enzyme for discriminating the target...
Designing a feasible primer pair is an important work before performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for single nucleotide polymorphism (SNP) genotyping. However, in many cases, no restriction enzymes are available to discriminate the target SNP, thus rendering the primer design useless. We propose a method that uses a genetic algorithm (GA) to search...
Many single nucleotide polymorphisms (SNPs) genotyping techniques have been developed but most of them are expensive. Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is a restriction enzyme-free and economic genotyping but its primer design is still computationally challenged. Here, we introduced a genetic algorithm (GA)-based PCR-CTPP primer design method. Thirty SNPs of the...
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