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Seven different two-photon excitable dipyrrylmetheneboron difluoride labels (dipyrrylmethene-BF2 labels) and a frequently used TAMRA label were conjugated to mouse IgG against α-fetoprotein in variable substitution degrees. Altogether 40 IgG conjugates were prepared, and studied with respect to one-photon absorption and emission properties, and two-photon fluorescence efficiency using 1064 nm laser...