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Key messageImprovingAgrobacterium-mediated transformation frequency and event quality by increasing binary plasmid copy number and appropriate strain selection is reported in an elite maize cultivar. Abstract Agrobacterium -mediated maize transformation is a well-established method for gene testing and for introducing...
Key messageAn improvedAgrobacterium-mediated transformation protocol is described for a recalcitrant commercial maize elite inbred with optimized media modifications and AGL1. These improvements can be applied to other commercial inbreds. Abstract This study describes a significantly improved Agrobacterium-mediated transformation protocol in a recalcitrant commercial maize elite inbred,...
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