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The roles of peroxisomes and microsomes on the biosynthetic pathway for docosahexaenoic acid (DHA) from α-linolenic acid (ALA) were investigated. Microsomes and peroxisomes were prepared from livers of fetal and neonatal piglets by a combination of differential and gradient layer centrifugation. Microsomes, peroxisomes, and combined cell fractions were incubated with [13C-U]18∶3n−3. The [M] and [M+18]...