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Twelve polymorphic microsatellite loci were developed for the Yellow-Striped Goatfish Mulloidichthys flavolineatus, from shallow shotgun pyrosequencing of genomic DNA. The 12 loci were characterized in a sample of 47 individuals from the Big Island of Hawai‘i, 21 individuals from Johnston Atoll (North Pacific) and 24 individuals from Okinawa (West Pacific). We found a mean of 8.08 ± 2.43 SEM alleles...