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The capture of circulating tumor cells (CTCs) in shear flow is studied systematically by driving suspensions of breast cancer cells, with different EpCAM receptor expression levels, through microchannels functionalized with EpCAM antibody ligands at various densities. The dependence of cell capture efficiency on flow rate is established and can be described by an exponential function; a characteristic...
The minimum detection limit in isolation of circulating tumor cells (CTCs) from binary mixtures is experimentally explored utilizing microchannel arrays functionalized with EpCAM antibodies. Hence, the tested binary mixtures contained EpCAM-positive and EpCAM-negative cells serving as target and non-target cells, respectively. The ratio of target to non-target cells in the mixtures ranged from 1:1...
CD4+CD25+ regulatory T cells have been characterized as a critical population of immunosuppressive cells. They play a crucial role in cancer progression by inhibiting the effector function of CD4+ or CD8+ T lymphocytes. However, whether regulatory T lymphocytes that expand during tumor progression can modulate dendritic cell function is unclear. To address this issue, we have evaluated the inhibitory...
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