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The absorption and fluorescence spectra of dimethyloxyluciferin (DMOL) and monomethyloxyluciferin (MMOL) were studied at pH 3.0–12.0 In the range of pH 3.0–8.0, the fluorescence spectrum of DMOL exhibits a maximum at λem = 639 nm. At higher pH values an additional emission maximum appears at λem = 500 nm (wavelength of excitation maximum λex = 350 nm), which intensity increases with time. It is shown...
Dissociation constants (Ks) in the pH range 6.5–9.0 for complexes of luciferin, dimethyloxyluciferin (DMOL), and monomethylluciferin (MMOL) with recombinant wild-type and mutant (His433Tyr) luciferases from the Luciola mingrelica firefly were determined by fluorescent titration. The protonated effectors were bound by the wild-type and mutant luciferases better than the nonprotonated ones. The affinity...
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