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The effects of Diethyl aminoethyl hexanoate (DAH) on one-step micro-propagation culture with leave, petiole, and root explants were investigated. It was found that adding DAH into basic media increased the one-step culture rates from about less than 40% to more than 50%. For leaf explants, the optimum DAH concentration is 0.08 mg·L-1; petiole, 0.32 mg·L-1, and root explant,...
Anthers containing microspores at the uninucleate stage were excised from capitula of field grown plants of purple coneflower, Echinacea purpurea, and cultured on medium conducive to callus growth. In callus induction cultures, N6 basal medium was more effective than Murashige and Skoog (MS), and a combination of benzyladenine (BA) at 2.22 µM with naphthaleneacetic acid (NAA) at 0.054 µM was more...
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