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High-density electrodes network (HDEN) demonstrates a flexible electroporation procedure for gene transfection in different cell types. In this study, the HDEN device shows its capacity as a great candidate in optimization of efficiency in non-viral induction of murine induced pluripotent stem cells (iPSCs) by demonstrating successful expression of different kinds of transgenes of fluorescent proteins...
This paper reported an on-chip sensing method to record the highest processing temperature that the structure experienced by using the autofluorescence of Parylene C. Temperature properties of Parylene C autofluorescence was studied, that with the increment of processing temperature, the fluorescence intensity kept increasing. Therefore, the Parylene C was deposited on the chip as a temperature indicating...
This paper reported a MEMS fabrication compatible, damage free method for in-process 3D morphology reconstruction of high aspect ratio microstructure. As a novel morphology tracer, Parylene C thin film was conformally deposited onto the structure and annealed at high temperature under N2. The autofluorescence of Parylene C was considerably enhanced by the annealing, which made it possible to image...
We have developed a novel microchip aiming to rapidly optimize of electric parameters for electroporation. The electrodes were designed as symmetrical hyperbolic formatted to generate linearly decrescent electric field. Cells were cultured on the microchip for adherent electroporation and in-situ observation. Exhibited diversely in cell viability and transfection efficiency, three areas were observed...
We report a parylene-based flexible electroporation chip for in vivo nucleic acid delivery. Benefited from the flexibility of parylene film, our chip fits the natural shape of the electroporated objects, thereby provides a uniform electric field over a large area. In addition, the subject is less likely to be harmed owing to the chip features, including good biocompatibility, less invasive electroporation...
With the development of microfabrication, micropatterns attract more and more attentions in cellular biology research. However, fabrication of micron annulus patterns with feature size of several micrometers by the traditional lift-off technique is still tricky. In this work, an aluminum-photoresist dual-layer lift-off process was developed to achieve a high yield and good reproducible gold micropattern...
Electroporation, in which electric pulses are applied to create transient pores in the cell membrane, is widely used to introduce biologically active molecules into cells. Here we report a novel electroporation chip with great performance and good compatibility with the standard multi-well plate used in biological research. We demonstrated a prototype chip integrating with a 12-well plate and successfully...
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