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Advances in genome sequencing have revolutionized biology by providing the molecular blueprints for thousands of living organisms. Yet, the functions of a large fraction, as much as one-half, of the component parts remain unknown even for the best understood organisms, including Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae. Here, we describe our development of comprehensive genomic...
Since genomic sequencing project launched, during in 1990s, biological research environments has been dramatically changed by developments of inter-disciplinary fields between biology and others such as chemistry, physics, information science, mathematics and engineering. Many high-throughput systems to obtain comprehensive analysis results have become available. As the result, accumulation of experimental...
Bacterial drug resistance is a serious concern for human health. Multidrug efflux pumps export a broad variety of substrates out of the cell and thereby convey resistance to the host. In Escherichia coli, the AcrB:AcrA:TolC efflux complex forms a principal transporter for which structures of the individual component proteins have been determined in isolation. Here, we present the X-ray structure of...
Reactions of 2,2-dibromo-2-fluoroethyl alkyl ethers and dibromofluoromethylcarbinyl silyl ethers with CrCl 2 and Mn powder provided 2-fluorovinyl alkyl ethers and 1-fluoro-2-alkenyl enol silyl ethers in high yields and in Z selective manner.
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