Search results for: Anna Nadal

Items from 1 to 18 out of 18 results

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Lack of adverse effects in subchronic and chronic toxicity/carcinogenicity studies on the glyphosate-resistant genetically modified maize NK603 in Wistar Han RCC rats

Pablo Steinberg, Hilko Voet, Paul W. Goedhart, Gijs Kleter, more

Archives of Toxicology > 2019 > 93 > 4 > 1095-1139

In 2012, a controversial study on the long-term toxicity of a Roundup herbicide and the glyphosate-tolerant genetically modified (GM) maize NK603 was published. The EC-funded G-TwYST research consortium tested the potential subchronic and chronic toxicity as well as the carcinogenicity of the glyphosate-resistant genetically modified maize NK603 by performing two 90-day feeding trials, one with GM...

article

Novel Rosaceae plant elicitor peptides as sustainable tools to control Xanthomonas arboricola pv. pruni in Prunus spp.

Cristina Ruiz, Anna Nadal, Emilio Montesinos, Maria Pla

Molecular Plant Pathology > 19 > 2 > 418 - 431

Fruit crops are regarded as important health promoters and constitute a major part of global agricultural production, and Rosaceae species are of high economic impact. Their culture is threatened by bacterial diseases, whose control is based on preventative treatments using compounds of limited efficacy and negative environmental impact. One of the most economically relevant examples is the pathogen...

article

Diversity of plant defense elicitor peptides within the Rosaceae

Cristina Ruiz, Anna Nadal, Laura Foix, Laura Montesinos, more

BMC Genetics > 2018 > 19 > 1 > 1-12

Background Plant elicitor peptides (Peps) are endogenous molecules that induce and amplify the first line of inducible plant defense, known as pattern-triggered immunity, contributing to protect plants against attack by bacteria, fungi and herbivores. Pep topic application and transgenic expression have been found to enhance disease resistance in a small number of model plant-pathogen systems. The...

article

Asynchronous Flowering or Buffer Zones: Technical Solutions for Small‐scale Farming

Anna Nadal, Maria Pla, Joaquima Messeguer, Enric Melé, more

EuroChoices > 15 > 1 > 24 - 30

To ensure consumers' and farmers' freedom of choice, many countries have regulated the labelling of products derived from genetically modified (GM) crops and coexistence of conventional and GM fields. On the basis of published research results a guide for Good Agricultural Practices (GAP) was compiled to facilitate maize coexistence in farming zones with temperate climate. In the frame of the PRICE...

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High CO₂ concentration as an inductor agent to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories

Cristina Ruiz, Maria Pla, Nuri Company, Jordi Riudavets, more

Plant Molecular Biology > 2016 > 90 > 4-5 > 329-343

Cationic α-helical antimicrobial peptides such as BP100 are of increasing interest for developing novel phytosanitary or therapeutic agents and products with industrial applications. Biotechnological production of these peptides in plants can be severely impaired due to the toxicity exerted on the host by high-level expression. This can be overcome by using inducible promoters with extremely low activity...

article

The production of recombinant cationic a-helical antimicrobial peptides in plant cells induces the formation of protein bodies derived from the endoplasmic reticulum

Cristina Ruiz Ramirez, Nuri Company, Anna Nadal, Jose-Luis La Paz, more

New BIOTECHNOLOGY > 2014 > 31 > Supplement > S195

article

The production of recombinant cationic α‐helical antimicrobial peptides in plant cells induces the formation of protein bodies derived from the endoplasmic reticulum

Nuri Company, Anna Nadal, José‐Luis La Paz, Sílvia Martínez, more

Plant Biotechnology Journal > 12 > 1 > 81 - 92

Synthetic linear antimicrobial peptides with cationic α‐helical structures, such as BP100, are valuable as novel therapeutics and preservatives. However, they tend to be toxic when expressed at high levels as recombinant peptides in plants, and they can be difficult to detect and isolate from complex plant tissues because they are strongly cationic and display low extinction coefficient and extremely...

article

Medium-term evaluation of an educational intervention on dietary and physical exercise habits in schoolchildren: The Avall 2 study

Esteve Llargués, Assumpta Recasens, Rosa Franco, Anna Nadal, more

Endocrinologia y nutricion (English Edition) > 2012 > 59 > 5 > 288-295

To assess whether the benefits seen in nutrition, physical activity and body mass index were maintained at 2 years of completion of the educational intervention.An experimental, longitudinal, 4-year, two-arm, parallel study with cluster randomization to assess an intervention program aimed at children in their first year of primary schooling attending schools in the city of Granollers. Intervention...

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Only half the transcriptomic differences between resistant genetically modified and conventional rice are associated with the transgene

Maria Montero, Anna Coll, Anna Nadal, Joaquima Messeguer, more

Plant Biotechnology Journal > 9 > 6 > 693 - 702

Besides the intended effects that give a genetically modified (GM) plant the desired trait, unintended differences between GM and non‐GM comparable plants may also occur. Profiling technologies allow their identification, and a number of examples demonstrating that unintended effects are limited and diverse have recently been reported. Both from the food safety aspect and for research purposes, it...

article

Proteomic analysis of MON810 and comparable non-GM maize varieties grown in agricultural fields

Anna Coll, Anna Nadal, Michel Rossignol, Pere Puigdomènech, more

Transgenic Research > 2011 > 20 > 4 > 939-949

Worldwide maize is the second major agricultural commodity and around one-fourth is currently biotech, with significant application of the insect resistant event MON810 particularly in the European Union. Grains are the major commercialized part of the plant, and can be harvested after maturity (for food and feed purposes) or at late milky-starchy stage (for forage uses, with the whole plant). We...

article

Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices

Anna Coll, Anna Nadal, Rosa Collado, Gemma Capellades, more

Plant Molecular Biology > 2010 > 73 > 3 > 349-362

The introduction of genetically modified organisms (GMO) in many countries follows strict regulations to ensure that only safety-tested products are marketed. Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation. Here we used a commercial (Affymertix) microarray platform (i.e. allowing assessing the expression...

article

Effect of volunteers on maize gene flow

Montserrat Palaudelmàs, Gisela Peñas, Enric Melé, Joan Serra, more

Transgenic Research > 2009 > 18 > 4 > 583-594

Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate areas, we investigated the real impact of GM volunteers on the yield of 12 non-GM...

article

Gene expression profiles of MON810 and comparable non-GM maize varieties cultured in the field are more similar than are those of conventional lines

Anna Coll, Anna Nadal, Rosa Collado, Gemma Capellades, more

Transgenic Research > 2009 > 18 > 5 > 801-808

Maize is a major food crop and genetically modified (GM) varieties represented 24% of the global production in 2007. Authorized GM organisms have been tested for human and environmental safety. We previously used microarrays to compare the transcriptome profiles of widely used commercial MON810 versus near-isogenic varieties and reported differential expression of a small set of sequences in leaves...

article

Lack of repeatable differential expression patterns between MON810 and comparable commercial varieties of maize

Anna Coll, Anna Nadal, Montserrat Palaudelmàs, Joaquima Messeguer, more

Plant Molecular Biology > 2008 > 68 > 1-2 > 105-117

The introduction of genetically modified organisms (GMO) in many countries follows strict regulations to assure that only products that have been safety tested in relation to human health and the environment are marketed. Thus, GMOs must be authorized before use. By complementing more targeted approaches, profiling methods can assess possible unintended effects of transformation. We used microarrays...

article

A molecular beacon-based real time NASBA assay for detection of Listeria monocytogenes in food products: Role of target mRNA secondary structure on NASBA design

Anna Nadal, Anna Coll, Nigel Cook, Maria Pla

Journal of Microbiological Methods > 2007 > 68 > 3 > 623-632

A molecular beacon-based real-time NASBA (QNASBA) assay for detection and identification of Listeria monocytogenes has been developed. A correlation between targeting highly accessible mRNA sequences and QNASBA efficiency and sensitivity was demonstrated. The assay targets a sequence from the mRNA transcript of the hly gene which is specific for this bacterium; and includes an internal amplification...

article

Catalytic RNase P RNA from Synechocystis sp. cleaves the hepatitis C virus RNA near the AUG start codon

Rosario Sabariegos, Anna Nadal, Nerea Beguiristain, Maria Piron, more

FEBS Letters > 2004 > 577 > 3 > 517-522

Previously, we described two RNA structural motifs in the hepatitis C viral (HCV) genome that can be processed in vitro by human ribonuclease P (RNase P) enzyme [J. Biol. Chem. 277 (2002) 30606]. One of these structures is located in the internal ribosome entry site and is conserved in the related animal pestiviruses [J. Biol. Chem. 278 (2003) 26844]. Here, we tested two prokaryotic RNase P ribozymes...

article

The 7472insC mtDNA mutation impairs 5′ and 3′ processing of tRNASer(UCN)

Marina Toompuu, Louis L. Levinger, Anna Nadal, Jordi Gomez, more

Biochemical and Biophysical Research Communications > 2004 > 322 > 3 > 803-813

The deafness-associated 7472insC mtDNA mutation was previously shown to decrease the steady-state level of tRNA ^Ser(UCN) post-transcriptionally. To identify the affected tRNA maturation step(s) we analysed the effects of the mutation on processing in vivo and in vitro. tRNA ^Ser(UCN) from cybrid cells homoplasmic for 7472insC contained a high frequency (>11%) of molecules misprocessed...

article

Stress proteins co-expressed in suberized and lignified cells and in apical meristems

Maria Pla, Gemma Huguet, Dolors Verdaguer, Pere Puigderrajols, more

Plant Science > 1998 > 139 > 1 > 49-57

We report the cloning of a small heat shock protein, Qs HSP17, and an osmotin like protein, Qs_OLP, from cork oak phellem tissue (cork cells). Both genes are expressed in suberizing cells and in other cells subject to endogenous stress associated with free radicals. We provide evidence that smHSPs and OLPs accumulate in overwintering buds and speculate that their role is similar to that in seed dormancy...

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