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A new imaging system allows visualizing oxygen distributions in 2D. Combining sensor foils with microfluidic devices enables online monitoring of cellular oxygen consumption in whole chip areas. Furthermore, suitable device materials depending on application and cell line can be determined. Numerically simulated oxygen consumption of rat lung microvascular endothelial cells and rat hepatocytes was...
There is great demand for constructing well-organized three-dimensional (3D) tissues in vitro. Here, we developed a 3D stacked culture method using biodegradable poly(d,l-lactide-co- glycolide) (PLGA) membranes with defined topography. Pore size and porosity of the membranes can be controlled by changing the moisture content during fabrication. The optimized membrane served as a scaffold to manipulate...
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