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Aims/hypothesis The interaction of syntaxin-1 and SNAP-25 with insulin exocytosis was examined using the diabetic Goto–Kakizaki (GK) rat and a total internal reflection fluorescence (TIRF) imaging system. Methods Primary rat pancreatic beta cells were immunostained with anti-syntaxin-1A, anti-SNAP-25 and anti-insulin antibodies, and then observed by TIRF microscopy. The real-time image of GFP-labelled...