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Single molecule localization microscopy has made great improvements in spatial resolution achieving performance beyond the diffraction limit by sequentially activating and imaging small subsets of molecules. Here, we present an algorithm designed for high-density molecule localization which is of a major importance in order to improve the temporal resolution of such microscopy techniques. We formulate...
This communication presents a pipeline for Multi-Angle TIRF calibration from the measurement of the incident angle to the model validation. This problem is of major importance when dealing with 3D reconstruction methods from a set of MA-TIRF acquisitions since the reconstruction accuracy highly depends on the agreement between the theoretical model and the physical system. One main issue is then to...
This paper is devoted to two classical sparse problems in array processing: Channel estimation and DOA estimation. It is shown after some background and some recent results in ℓ0 optimization how this latter can be used, at the same computational cost, in order to obtain improvement in comparison with ℓ1 optimization for sparse estimation.
In this paper we address the problem of cells detection from microscopy images. We construct a dictionary of candidate shapes obtained from previous segmentation maps and define an energy function to select the best candidates. The energy minimization is performed by an iterative graph cut algorithm. The proposed approach optimally combines the segmentation maps obtained with different methods and/or...
Super-resolution microscopy techniques allow to overstep the diffraction limit of conventional optics. Theses techniques are very promising since they give access to the visualisation of finer structures which is of fundamental importance in biology. In this paper we deal with Multiple-Angle Total Internal Reflection Microscopy (MA-TIRFM) which allows to reconstruct 3D sub-cellular structures of a...
We propose a new model for the reconstruction of biological structures using Multiple-Angle Total Internal Reflection Fluorescence Microscopy (MA-TIRFM). This recent microscopy technique allows the visualization of sub-cellular structures around the plasma membrane which is of fundamental importance in the comprehension of exchanges mechanisms of the cell. We present a 3D reconstruction method based...
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