Search results for: Jun-Fu Huang

Items from 1 to 14 out of 14 results

chapter

A comprehensive solution for BEOL variation characterization and modeling

Katherine Chiang, Jun-Fu Huang, Tai-Yu Cheng, Cheng Hsiao, more

2016 International Conference on Simulation of Semiconductor Processes and Devices (SISPAD) > 307 - 310

2016 International Conference on Simulation of Semiconductor Processes and Devices (SISPAD)

A comprehensive variation model is critical to achieve both competitive design and manufacturing yield in advanced technologies. Conventionally, as long as FEOL (front end of line) statistical model is appropriate, BEOL (back end of line) variations given by lumping multiple variation sources into few corners is enough to achieve reliable simulation results. However, as BEOL contribution is becoming...

article

Sensitive and specific detection of miRNA using an isothermal exponential amplification method using fluorescence-labeled LNA/DNA chimera primers

Jun-Fu Huang, Na Zhao, Han-Qing Xu, Han Xia, more

Analytical and Bioanalytical Chemistry > 2016 > 408 > 26 > 7437-7446

MicroRNAs (miRNAs) are currently considered as potential biomarkers for various human diseases. In the present study, miRNA-triggered real-time fluorescent isothermal reaction with exponential amplification (ReFIRE) with or without Thermus aquaticus MutS (Taq MutS) was developed to analyze miRNAs using DNA polymerase, a nicking endonuclease, and fluorescently labeled primers. In the absence of Taq...

article

Effect of moisture on electrical properties and reliability of low dielectric constant materials

Yi-Lung Cheng, Ka-Wai Leon, Jun-Fu Huang, Wei-Yuan Chang, more

Microelectronic Engineering > 2014 > 114 > Complete > 12-16

The effect of absorbed moisture on the electrical characteristics and reliability of low dielectric constant materials (low-k) was investigated in this study. The experimental results reveal that porous low-k dielectrics absorb more moisture than dense low-k dielectrics. This absorbed moisture degrades the electrical performance and reliability of both classes of low-k dielectrics. Annealing at a...

article

High-sensitivity PCR method for detecting BRAF V600Emutations in metastatic colorectal cancer using LNA/DNA chimeras to block wild-type alleles

Dong Chen, Jun-Fu Huang, Han Xia, Guang-Jie Duan, more

Analytical and Bioanalytical Chemistry > 2014 > 406 > 9-10 > 2477-2487

The response to epidermal growth factor receptor (EGFR)-targeted therapy in metastatic colorectal cancer (mCRC) is variable because of intra-tumor heterogeneity at the genetic level, and consequently, it is important to develop sensitive and selective assays to predict patient responses to therapy. Low-abundance BRAF V600E mutations are associated with poor response to treatment with EGFR inhibitors...

article

Impact of plasma treatment on structure and electrical properties of porous low dielectric constant SiCOH material

Yi-Lung Cheng, Jun-Fu Huang, Yu-Min Chang, Jihperng Leu

Thin Solid Films > 2013 > 544 > Complete > 537-540

Low dielectric constant (low-k) porous films are needed for advanced technologies to improve signal propagation. The integration of porous low-k films faces more severe challenges due to the presence of porosity. Plasma treatments have been considered to be critical steps to impact the low-k films' properties. In this study, the effect of various H₂/He plasma treatments on the porous low-k dielectrics...

article

Effect of thermal treatment on physical, electrical properties and reliability of porogen-containing and porogen-free ultralow-k dielectrics

Yu-Min Chang, Wei-Yuan Chang, Jun-Fu Huang, Jihperng Leu, more

Thin Solid Films > 2013 > 528 > Complete > 67-71

The effects of thermal annealing on the physical, electrical properties and reliability of porogen-containing and porogen-free ultralow-k dielectrics prepared by plasma-enhanced chemical vapor deposition (PECVD) are investigated. The porogen-free low-k dielectrics are obtained by using UV curing process to removal organic sacrificial phase and to generate open porosity. The results are compared with...

article

Species-specific identification of ruminant components contaminating industrial crude porcine heparin using real-time fluorescent qualitative and quantitative PCR

Qing Huang, Ting Xu, Gui-Yu Wang, Jun-Fu Huang, more

Analytical and Bioanalytical Chemistry > 2012 > 402 > 4 > 1625-1634

Ever since the emergence of bovine spongiform encephalopathy, the source of pharmaceutical heparin has been restricted to porcine intestinal mucosa. In this project, two real-time fluorescent PCR methods were developed to assist with quality control analysis. The first is a qualitative method which relies on SYBR Green I chemistry to confirm the porcine origin of industrial crude porcine heparin (ICPH),...

article

High sensitive mutation analysis on KRAS2 gene using LNA/DNA chimeras as PCR amplification blockers of wild-type alleles

Qing Huang, Gui-Yu Wang, Jun-Fu Huang, Bo Zhang, more

Molecular and Cellular Probes > 2010 > 24 > 6 > 376-380

The missense mutations at codons 12 and 13 of KRAS gene have been confirmed as a predictor of nonresponse to EGFR-targeted therapy with monoclonal antibodies cetuximab and panitumumab in patients with metastatic colorectal carcinoma (mCRC). Because of the intra-tumor heterogeneity at genetic levels, it is important to develop sensitive and selective assays to detect above KRAS mutation of rare mutated...

article

Rapid and Simultaneous Determination of Essential Minerals and Trace Elements in Human Milk by Improved Flame Atomic Absorption Spectroscopy (FAAS) with Microwave Digestion

Yang Luo, Bo Zhang, Ming Chen, Jue Wang, more

Journal of Agricultural and Food Chemistry > 2010 > 58 > 17 > 9396 - 9400

article

Isolation and enrichment of human genomic CpG islands by methylation-sensitive mirror orientation selection

Qing Huang, Larry Baum, Jun-Fu Huang, Jian-Ping You, more

Analytical Biochemistry > 2007 > 365 > 2 > 153-164

CpG islands (CGIs) in human genomic DNA are GC-rich fragments whose aberrant methylation is associated with human disease development. In the current study, methylation-sensitive mirror orientation selection (MS–MOS) was developed to efficiently isolate and enrich unmethylated CGIs from human genomic DNA. The unmethylated CGIs prepared by the MS–MOS procedure subsequently were used to construct a...

chapter

Modeling Sub-90nm On-Chip Variation Using Monte Carlo Method for DFM

Jun-Fu Huang, Changm VCY, S. Liu, K.Y.Y. Doong, more

2007 Asia and South Pacific Design Automation Conference > 221 - 225

2007 Asia and South Pacific Design Automation Conference

For sub-90nm technology nodes and below, random fluctuations of within-die physical process properties are also known as random on-chip variation (OCV). It impacts on the VLSI/SoC design yields significantly. This paper presents a recent silicon test chip experiment result which uses a set of innovative nanometer test structures and Monte-Carlo-based three-dimensional electromagnetic RC simulations...

article

Species-specific identification by inhibitor-controlled PCR of ruminant components contaminating industrial crude porcine heparin

Qing Huang, Chun-Yan Yao, Bin Chen, Feng Wang, more

Molecular and Cellular Probes > 2006 > 20 > 3-4 > 250-258

With the emergence of bovine spongiform encephalopathy, the source of pharmaceutical heparin is currently restricted to porcine intestinal mucosa. To control the species origin of industrial crude heparin, molecular biology methods relying on species-specific protein or DNA analysis should be developed to identify the ruminant components that might be contaminants in industrial crude porcine heparin...

article

Inactivation of dengue virus by methylene blue/narrow bandwidth light system

Qing Huang, Wei-Ling Fu, Bing Chen, Jun-Fu Huang, more

Journal of Photochemistry & Photobiology, B: Biology > 2004 > 77 > 1-3 > 39-43

Peracetic acid was one of the most commonly used disinfectants on solid surfaces in hospitals or public places. However, peracetic acid is an environmental toxin. Therefore, safer, alternative disinfectants or disinfectant systems should be developed. Because photodynamic virus inactivation with methylene blue (MB)/light system has proven effective in blood banking, MB was selected as a photosensitizing...

article

Relative mRNA expression of the lactate dehydrogenase A and B subunits as determined by simultaneous amplification and single strand conformation polymorphism - Relation with subunit enzyme activity

Ze-Jun Liu, Wei-Cong Peng, Xin Yang, Jun-Fu Huang, more

Journal of Chromatography B > 2003 > 793 > 2 > 405-412

To explore if it is correlated in human tumor cells that the expression of LDH homologous gene and LDH isoenzymes, we used RT-PCR-SSCP technique to measure the relative expression of genes with homologous sequences. The combination of PCR using common primers designed in the highly conserved regions and single-strand conformation polymorphism analysis of the products is used for quantitative determination...

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