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In article number 2000584, Milan Mrksich, Horacio D. Espinosa, and co‐workers report on a method for non‐destructively sampling enzymes multiple times from small populations of cells, using localized electroporation in microfluidic live cell analysis devices. The enzymes are captured in extraction chambers, where they modify immobilized substrates on a self‐assembled monolayer, and their activity...
Measuring changes in enzymatic activity over time from small numbers of cells remains a significant technical challenge. In this work, a method for sampling the cytoplasm of cells is introduced to extract enzymes and measure their activity at multiple time points. A microfluidic device, termed the live cell analysis device (LCAD), is designed, where cells are cultured in microwell arrays fabricated...
The transfection of molecules into cultured cells is a critical step toward fundamental studies of cells, drug screening, and stem cell reprogramming/differentiation. Despite rapid development of transfection methods, efficient transfection of adherent cells while maintaining viability is still challenging, in particular, for sensitive primary cells. To achieve this goal, we present a microfluidic...
A biologically inspired, multilayer laminate structural design is deployed into nanocomposite films of graphene oxide‐poly(methyl methacrylate) (GO‐PMMA). The resulting multilayer GO‐PMMA films show greatly enhanced mechanical properties compared to pure‐graphene‐oxide films, with up to 100% increases in stiffness and strength when optimized. Notably, a new morphology is observed at fracture surfaces:...
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