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To investigate the expression of CP in Down syndrome (DS) mouse model, we especially observed the changes in neuronal CP. We systematically analyzed the level of CP in Ts65Dn mouse, including serum CP concentration and enzymatic activity, CP mRNA in brain, the expression of CP protein in brain. The applied technologies were ELISA, chemical colorimetry, RT-PCR, immunohistochemistry. Compared with the...
The mutation detections of KRAS and BRAF genes are of significant importance to predict the responses to anti-cancer therapy and develop new drugs. In this paper, we developed a multi-step fluorescence resonance energy transfer (FRET) assay for multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers (CCP). The newly established detection system could detect as low as 2% mutant...
The combustion in circulated fluidized-bed (CFB) boiler is a complex and multi-variables coupling process. It is too difficult to build an exact mathematic model to describe the process. The main steam pressure control is an important subsystem of burning system, and it is difficult for the norm PID algorithm to control it. According to these, the human-simulated intelligent control technique is applied...
According to the production process and the control requirement of pure low temperature waste heat power generation plant, we design a set of distributed control system in waste heat power generation plant. The whole structure, task assignment and important control loop are analyzed. The system is composed of a supervisory and control layer and a process control layer. The DCS can realize the supervising...
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