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The trehalase (TRE) is one of enzymes related to trehalose catabolism in plant. On the basis of previous experiments, we are trying to inhibit the expression of trehalase gene through RNA interference technology in order to achieve the purpose of increasing the accumulation of trehalose in plant. So the fragments of TRE gene and RNAi-TRE have been obtained from Arabidopsis seedling by using RT-PCR...
The trehalose-6-phosphate phosphatase (TPP) is one of enzymes related to trehalose biosynthesis. But the researches of TPP were mainly focused in microorganism; few in the plant were reported. In this study, the TPP gene was cloned from Arabidopsis seedling by using RT-PCR (reverse transcription PCR) method. Then it was constructed to pET30a (+) vector and overexpressed in E.coli. Subsequently, the...
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